PAPOLG Polyclonal antibody, PBS Only

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• PAPOLG Polyclonal antibody, PBS Only (24284-1-PBS)

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  WB analysis of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 24284-1-AP (same clone as 24284-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 24284-1-AP (same clone as 24284-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

  IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 24284-1-AP (same clone as 24284-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PAPOLG antibody (24284-1-AP) at dilution of 1:1600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

PAPOLG Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 24284-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

24284-1-PBS targets PAPOLG in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16208

  Product name: Recombinant human PAPOLG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 469-736 aa of BC111701

  Sequence: QANNINMLKEGMKIEATHVKKKQLHHYLPAEILQKKKKQSLSDVNRSSGGLQSKRLSLDSSCLDSSRDTDNGTPFNSPASKSDSPSVGETERNSAEPAAVIVEKPLSVPPAQGLSIPVIGAKVDSTVKTVSPPTVCTIPTVVGRNVIPRITTPHNPAQGQPHLNGMSNITKTVTPKRSHSPSIDGTPKRLKDVEKFIRLESTFKDPRTAEERKRKSVDAIGGESMPIPTIDTSRKKRLPSKELPDSSSPVPANNIRVIKNSIRLTLNR

• Full Name: poly(A) polymerase gamma
• Calculated Molecular Weight: 736 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC111701
• Gene Symbol: PAPOLG
• Gene ID (NCBI): 64895
• RRID: AB_2879471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BWT3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.