PAPSS1 Polyclonal antibody

PAPSS1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 14708-1-AP

Synonyms

APS kinase, ATP sulfurylase, ATPSK1, PAPS synthase 1, PAPSS, PAPSS 1, PAPSS1, SAT Adenylyl sulfate kinase, SK 1, SK1, Sulfate adenylate transferase, Sulfurylase kinase 1



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14708-1-AP targets PAPSS1 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Chinese Hamster, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAPSS1 fusion protein Ag6401
Full Name 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Calculated molecular weight 71 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC050627
Gene symbol PAPSS1
Gene ID (NCBI) 9061
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PAPSS1 antibody 14708-1-APDownload protocol
IHC protocol for PAPSS1 antibody 14708-1-APDownload protocol
IF protocol for PAPSS1 antibody 14708-1-APDownload protocol
IP protocol for PAPSS1 antibody 14708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
Chinese HamsterWB

Biotechnol J

Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation.

Authors - Ren Liu
humanIF,CoIP

Cancer Res

CIB2 negatively regulates oncogenic signaling in ovarian cancer via sphingosine kinase 1.

Authors - Wenying Zhu