PAPSS1 Polyclonal antibody

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• PAPSS1 Polyclonal antibody (14708-1-AP)

PAPSS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14708-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

3'-phosphoadenosine-5'-phosphosulfate synthase, Adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenylylsulfate 3'-phosphotransferase, Adenylyl-sulfate kinase, APS kinase

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  WB analysis of HepG2 using 14708-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 14708-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 14708-1-AP

  IP result of anti-PAPSS1 (IP:14708-1-AP, 4ug; Detection:14708-1-AP 1:1000) with HepG2 cells lysate 1000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 14708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14708-1-AP (PAPSS1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 14708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14708-1-AP (PAPSS1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 14708-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 14708-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 14708-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat salivary gland using 14708-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 14708-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAPSS1 antibody (14708-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human ovary tumor tissue, rat salivary gland tissue, human testis tissue, human placenta tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14708-1-AP targets PAPSS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6401

  Product name: Recombinant human PAPSS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-624 aa of BC050627

  Sequence: MEIPGSLCKKVKLSNNAQNWGMQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKA

• Full Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC050627
• Gene Symbol: PAPSS1
• Gene ID (NCBI): 9061
• RRID: AB_2159487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43252
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The PAPSS1 Polyclonal antibody (Cat# 14708-1-AP) has 7 citations published in Nature, Cancer Research, Cell Reports Medicine, Frontiers in Molecular Biosciences, Biotechnology Journal, Histology and Histopathology, and bioRxiv. Research fields span cancer biology (ferroptosis, ovarian cancer, esophageal squamous carcinoma), neuroprotection in ischemic stroke, reproductive medicine (endometriosis), and antibody biotechnology.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Glycosaminoglycan-driven lipoprotein uptake protects tumours from ferroptosis; Authors - Dylan Calhoon
human	IF,CoIP	Cancer Res; CIB2 negatively regulates oncogenic signaling in ovarian cancer via sphingosine kinase 1.; Authors - Wenying Zhu
mouse,human	WB	Cell Rep Med; Neuroprotective response against the onset of ischemic stroke by upregulation of histone H3Y99 sulfation.; Authors - Li Jiang ; KD Validated
human	IHC	Front Mol Biosci; Potential biomarkers and immune infiltration linking endometriosis with recurrent pregnancy loss based on bioinformatics and machine learning; Authors - Jianhui Chen
hamster	WB	Biotechnol J; Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation.; Authors - Ren Liu
human	IHC	Histol Histopathol; Correlation of 3'-Phosphoadenosine-5'-Phosphosulfate synthase 1 (PAPSS1) expression with clinical parameters and prognosis in esophageal squamous carcinoma; Authors - Xuyou Zhu