PARP11 Polyclonal antibody

PARP11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16692-1-AP

Synonyms

C12orf6, PARP 11, PARP11

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Validation Data Gallery

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  WB analysis of PC-3 using 16692-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, mouse heart tissue, mouse spleen tissue
• Positive IHC detected in: human kidney tissue, human placenta tissue, human colon tissue, human testis tissue, human skin tissue, human liver tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16692-1-AP targets PARP11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARP11 fusion protein Ag10053
• Full Name: poly (ADP-ribose) polymerase family, member 11
• Calculated Molecular Weight: 331 aa, 39 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC017569
• Gene Symbol: PARP11
• Gene ID (NCBI): 57097
• RRID: AB_2158504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP11, also named as ARTD11, belongs to the  ARTD/PARP family. PARP11 is a mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. PARP11 plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (PMID: 25043379). PARP11 has 4 isoforms with the molecular mass of 28-40 kDa.