PARP2 Polyclonal antibody, PBS Only

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• PARP2 Polyclonal antibody, PBS Only (55149-1-PBS)

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  WB analysis using 55149-1-AP (same clone as 55149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55149-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 55149-1-AP (same clone as 55149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55149-1-AP (PARP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 55149-1-AP (same clone as 55149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55149-1-AP (PARP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 55149-1-AP (same clone as 55149-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55149-1-AP (PARP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

PARP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 55149-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ADPRT2, ADPRTL3, ARTD2, DNA ADP-ribosyltransferase PARP2, EC:2.4.2.-

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Product Information

55149-1-PBS targets PARP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: poly (ADP-ribose) polymerase 2
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_005484
• Gene Symbol: PARP2
• Gene ID (NCBI): 10038
• RRID: AB_10858796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UGN5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).