PARP4 Polyclonal antibody, PBS Only

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• PARP4 Polyclonal antibody, PBS Only (19775-1-PBS)

• 

  WB analysis using 19775-1-AP (same clone as 19775-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 19775-1-AP (PARP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 19775-1-AP (same clone as 19775-1-PBS)

  WB result of PARP4 antibody (19775-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARP4 transfected A549 cells. This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19775-1-AP (same clone as 19775-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19775-1-AP (PARP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 19775-1-AP (same clone as 19775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19775-1-AP (PARP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 19775-1-AP (same clone as 19775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19775-1-AP (PARP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19775-1-AP (same clone as 19775-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using PARP4 antibody (19775-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PARP4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 19775-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 4, ADPRTL1, ARTD4, EC:2.4.2.-, p193

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Product Information

19775-1-PBS targets PARP4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: poly (ADP-ribose) polymerase family, member 4
• Calculated Molecular Weight: 193 kDa
• Observed Molecular Weight: 193 kDa
• GenBank Accession Number: NM_006437
• Gene Symbol: PARP4
• Gene ID (NCBI): 143
• RRID: AB_3085585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKK3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PARP4, also named as ADPRTL1, KIAA0177, PARPL, VPARP and PH5P, catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.