PE-conjugated PAX6 Recombinant antibody

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  FC experiment of U-251 using PE-84345-5

  1x10^6 U-251 cells were intracellularly stained with 5 ul PE Pax6 Recombinant Antibody (PE-84345-5, Clone:241521G1)(red), or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of HeLa using PE-84345-5

  1x10^6 HeLa cells were intracellularly stained with 5 ul PE Pax6 Recombinant Antibody (PE-84345-5, Clone:241521G1)(red), or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

PAX6 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. PE-84345-5

Clone No.241521G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

241521G1, paired box 6, Paired box protein Pax 6, Paired box protein Pax-6, PAX 6

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Tested Applications

• Positive FC (Intra) detected in: U-251 cells, HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-84345-5 targets PAX6 in FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PAX6 fusion protein Ag2984
• Full Name: paired box 6
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC011953
• Gene Symbol: PAX6
• Gene ID (NCBI): 5080
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26367
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.