Tested Applications
Positive FC (Intra) detected in | U-251 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
PE-84345-5 targets PAX6 in FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PAX6 fusion protein Ag2984 Predict reactive species |
Full Name | paired box 6 |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 47 kDa |
GenBank Accession Number | BC011953 |
Gene Symbol | PAX6 |
Gene ID (NCBI) | 5080 |
Conjugate | PE Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P26367 |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.