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PBRM1 Polyclonal antibody, PBS Only (12563-1-PBS)

Filter: AllWBIPIHC

WB analysis of Jurkat using 12563-1-AP (same clone as 12563-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 12563-1-AP (PBRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12563-1-PBS in a different storage buffer formulation.
IP experiment of Jurkat using 12563-1-AP (same clone as 12563-1-PBS)
IP result of anti-PBRM1 (IP:12563-1-AP, 4ug; Detection:12563-1-AP 1:8000) with Jurkat cells lysate 1280 ug. This data was developed using the same antibody clone with 12563-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 12563-1-AP (same clone as 12563-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12563-1-AP (PBRM1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12563-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 12563-1-AP (same clone as 12563-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12563-1-AP (PBRM1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12563-1-PBS in a different storage buffer formulation.

PBRM1 Polyclonal antibody, PBS Only

Name: PBRM1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12563-1-PBS

PBRM1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12563-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

BAF180, BRG1-associated factor 180, hPB1, PB1, PBRM 1

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12563-1-PBS targets PBRM1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3148 Product name: Recombinant human PBRM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC015323 Sequence: MGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV Predict reactive species
Full Name	polybromo 1
Calculated Molecular Weight	180 kDa
Observed Molecular Weight	180-195 kDa, 100 kDa
GenBank Accession Number	BC015323
Gene Symbol	PBRM1
Gene ID (NCBI)	55193
RRID	AB_2877865
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86U86
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PBRM1, also named hPB1 BAF180 and Polybromo-1D, acts as a negative regulator of cell proliferation. PBRM1 is the second major mutated clear cell RCC cancer gene after VHL. PBRM1 loss is associated with low grade features.