PCBP3 Recombinant monoclonal antibody

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  WB analysis using 85180-3-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 85180-3-RR (PCBP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85180-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85180-3-RR against Human PCBP3 were performed. The affinity constant is below 1 pM.

PCBP3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85180-3-RR

Clone No.242791F11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242791F11, ALPHA CP3, Alpha-CP3, PCBP3OT, PCBP3-OT1

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Tested Applications

• Positive WB detected in: MCF-7 cells, K-562 cells, IMR-32 cells, RAW 264.7 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85180-3-RR targets PCBP3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9202

  Product name: Recombinant human PCBP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-313 aa of BC012061

  Sequence: MESKVSEGGLNVTLTIRLLMHGKEVGSIIGKKGETVKKMREESGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSPATSKPPVTLRLVVPASQCGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLEAYTIQGQYAIPHPDLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEEAQNLMGQSSGLDASPPASTHELTIPNDLIGCIIGRQGTKINEIRQMSGAQIKIANATEGSSERQITITGTPANISLAQYLINARLTSEVTGMGTL

• Full Name: poly(rC) binding protein 3
• Calculated Molecular Weight: 313 aa, 33 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC012061
• Gene Symbol: PCBP3
• Gene ID (NCBI): 54039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P57721
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCBP3 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. PCBP3 is broadly expressed in various tissues, including the testis and endometrium. It has been associated with diseases such as dementia.