PCCB Monoclonal antibody

PCCB Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, mouse, human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A9A5

Cat no : 66501-1-Ig

Synonyms

PCCase subunit beta, PCCB



Tested Applications

Positive WB detected inJurkat cells, human heart tissue, HepG2 cells, HSC-T6 cells, pig heart tissue, rat heart tissue, mouse liver tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66501-1-Ig targets PCCB in WB, IHC, IF, ELISA applications and shows reactivity with pig, rat, mouse, human samples.

Tested Reactivity pig, rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCCB fusion protein Ag1608
Full Name propionyl Coenzyme A carboxylase, beta polypeptide
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC013768
Gene symbol PCCB
Gene ID (NCBI) 5096
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCCB antibody 66501-1-IgDownload protocol
IHC protocol for PCCB antibody 66501-1-IgDownload protocol
IF protocol for PCCB antibody 66501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols