PCDHA2 Polyclonal antibody, PBS Only

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• PCDHA2 Polyclonal antibody, PBS Only (10127-2-PBS)

• 

  WB analysis using 10127-2-AP (same clone as 10127-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 10127-2-AP (same clone as 10127-2-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 10127-2-AP (same clone as 10127-2-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10127-2-AP (same clone as 10127-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 10127-2-AP (same clone as 10127-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 10127-2-AP (same clone as 10127-2-PBS)

  IP result of anti-PCDHA2 (IP:10127-2-AP, 3ug; Detection:10127-2-AP 1:300) with HeLa cells lysate 4650ug. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

PCDHA2 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 10127-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

10127-2-PBS targets PCDHA2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0173

  Product name: Recombinant human PCDHA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 98-259 aa of BC003126

  Sequence: RSAECSIHVEVIVDRPLQVFHVEVEVKDINDNPPIFPMTVKTIRFPESRLLDSRFPLEGASDADIGVNALLSYKLSSSEFFFLDIQANDELSESLSLVLGKSLDREETAEVNLLLVATDGGKPELTGTVQILIKVLDVNDNEPTFAQSVYKVKLLENTANGT

• Full Name: protocadherin alpha 2
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 102 kDa, 80 kDa
• GenBank Accession Number: BC003126
• Gene Symbol: PCDHA2
• Gene ID (NCBI): 56146
• RRID: AB_2158964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5H9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA2 (protocadherin alpha 2) is a single-pass type I membrane protein containing 6 cadherin domains. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. Three isoforms produced by alternative splicing have been described (UniProt).