PCIF1 Polyclonal antibody, PBS Only

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• PCIF1 Polyclonal antibody, PBS Only (16082-1-PBS)

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  WB analysis using 16082-1-AP (same clone as 16082-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16082-1-AP (same clone as 16082-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 16082-1-AP (same clone as 16082-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 16082-1-AP (same clone as 16082-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16082-1-AP (same clone as 16082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16082-1-AP (same clone as 16082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 16082-1-AP (same clone as 16082-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 16082-1-AP (same clone as 16082-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

PCIF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16082-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C20orf67, CAPAM, Cap-specific adenosine methyltransferase, EC:2.1.1.62, hCAPAM

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Product Information

16082-1-PBS targets PCIF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9080

  Product name: Recombinant human PCIF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 355-704 aa of BC013365

  Sequence: DSVEGICSKIYHISLEYVKRIREKHLAILKENNISEEVEAPEVEPRLVYCYPVRLAVSAPPMPSVEMHMENNVVCIRYKGEMVKVSRNYFSKLWLLYRYSCIDDSAFERFLPRVWCLLRRYQMMFGVGLYEGTGLQGSLPVHVFEALHRLFGVSFECFASPLNCYFRQYCSAFPDTDGYFGSRGPCLDFAPLSGSFEANPPFCEELMDAMVSHFERLLESSPEPLSFIVFIPEWREPPTPALTRMEQSRFKRHQLILPAFEHEYRSGSQHICKKEEMHYKAVHNTAVLFLQNDPGFAKWAPTPERLQELSAAYRQSGRSHSSGSSSSSSSEAKDRDSGREQGPSREPHPT

• Full Name: PDX1 C-terminal inhibiting factor 1
• Calculated Molecular Weight: 704 aa, 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC013365
• Gene Symbol: PCIF1
• Gene ID (NCBI): 63935
• RRID: AB_2159996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4Z3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.