PCK2 Monoclonal antibody, PBS Only

• 

  WB analysis using 67676-1-Ig (same clone as 67676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67676-1-Ig (same clone as 67676-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 67676-1-Ig (same clone as 67676-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit liver using 67676-1-Ig (same clone as 67676-1-PBS)

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 67676-1-Ig (same clone as 67676-1-PBS)

  IP result of anti-PCK2 (IP:67676-1-Ig, 4ug; Detection:67676-1-Ig 1:2000) with HepG2 cells lysate 1640 ug. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCK2 antibody (67676-1-Ig, Clone: 1B3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

PCK2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 67676-1-PBS

Clone No.1B3F1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

1B3F1, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M

---

Product Information

67676-1-PBS targets PCK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7021

  Product name: Recombinant human PCK2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 292-640 aa of BC001454

  Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

• Full Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC001454
• Gene Symbol: PCK2
• Gene ID (NCBI): 5106
• RRID: AB_2882871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q16822
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.