CoraLite® Plus 488-conjugated PCM1 Recombinant antibody

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  IF Staining of HeLa using CL488-84979

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PCM1 antibody (CL488-84979, Clone: 242199A9 ) at dilution of 1:200.

PCM1 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84979

Clone No.242199A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

242199A9, hPCM 1, hPCM-1, PCM 1, PCM-1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84979 targets PCM1 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PCM1 fusion protein Ag13977
• Full Name: pericentriolar material 1
• Calculated Molecular Weight: 2024 aa, 228 kDa
• Observed Molecular Weight: 245-320 kDa
• GenBank Accession Number: BC000453
• Gene Symbol: PCM1
• Gene ID (NCBI): 5108
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15154
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).