PCSK1 Polyclonal antibody

PCSK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28219-1-AP

Synonyms

BMIQ12, NEC 1, NEC1, Neuroendocrine convertase 1, PC1, PC3, PCSK1, Prohormone convertase 1, Proprotein convertase 1, SPC3

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Validation Data Gallery

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  WB analysis using 28219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28219-1-AP (PCSK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells
• Positive IHC detected in: human pancreas tissue, mouse pancreas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

28219-1-AP targets PCSK1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

• Tested Reactivity: Human, Mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCSK1 fusion protein Ag28203
• Full Name: proprotein convertase subtilisin/kexin type 1
• Calculated Molecular Weight: 753 aa, 84 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC130295
• Gene Symbol: PCSK1
• Gene ID (NCBI): 5122
• RRID: AB_2881093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein.

Publications

Species	Application	Title
mouse	WB	Mol Nutr Food Res; The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation; Authors - Mengcheng Jin
human	IHC	Rapid Commun Mass Spectrom; Data-independent acquisition combined with liquid chromatography mass spectrometry technique to detect prognostic protein markers in type I gastric neuroendocrine neoplasm; Authors - Meng Zhang