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PCSK1 Polyclonal antibody, PBS Only (28219-1-PBS)

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WB analysis using 28219-1-AP (same clone as 28219-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28219-1-AP (PCSK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 28219-1-AP (same clone as 28219-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 28219-1-AP (same clone as 28219-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 28219-1-AP (same clone as 28219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 28219-1-AP (same clone as 28219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 28219-1-AP (same clone as 28219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 28219-1-AP (same clone as 28219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

PCSK1 Polyclonal antibody, PBS Only

Name: PCSK1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 28219-1-PBS

PCSK1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28219-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

28219-1-PBS targets PCSK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag28203 Product name: Recombinant human PCSK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 406-585 aa of BC130295 Sequence: DMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIV Predict reactive species
Full Name	proprotein convertase subtilisin/kexin type 1
Calculated Molecular Weight	753 aa, 84 kDa
Observed Molecular Weight	66 kDa
GenBank Accession Number	BC130295
Gene Symbol	PCSK1
Gene ID (NCBI)	5122
RRID	AB_2881093
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P29120
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein.