CoraLite® Plus 647 Anti-Mouse PD-1/CD279 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL647-98080

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with FITC Plus Anti-Mouse CD3 and 0.25 ug CoraLite® Plus 647 Anti-Mouse PD-1/CD279 Rabbit RecAb (CL647-98080, Clone:240389B8). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-98080

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse PD-1/CD279 Rabbit RecAb (CL647-98080, Clone:240389B8) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse PD-1/CD279 Rabbit RecAb (CL647-98080, Clone:240389B8) (black, dashed). Cells were not fixed.

PD-1/CD279 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98080

Clone No.240389B8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD279, PD-1, PD1, Pdc1, PD 1

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Tested Applications

• Positive FC detected in: anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98080 targets PD-1/CD279 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: programmed cell death 1
• Calculated Molecular Weight: 32 kDa
• GenBank Accession Number: NM_008798.2
• Gene Symbol: PD-1
• Gene ID (NCBI): 18566
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02242
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).