Tested Applications
| Positive FC detected in | anti-CD3/CD28 treated mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CLV510-65684-2 targets PD-1/CD279 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Mouse PD-1 transfected BHK cells Predict reactive species |
| Full Name | programmed cell death 1 |
| GenBank Accession Number | BC119179 |
| Gene Symbol | PD-1 |
| Gene ID (NCBI) | 18566 |
| Conjugate | CoraLux Violet 510 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 408 nm / 496 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). RMP1-14 is a monoclonal antibody that targets the murine PD-1 protein and can be used in mouse models to block the reaction between PD-1 and PD-L1/PD-L2 (PMID: 14764726; 31832706).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CoraLux Violet 510 PD-1/CD279 antibody CLV510-65684-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
