Anti-Human PD-L1 (B7-H1) (29E.2A3)

PD-L1 (B7-H1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

29E.2A3

Cat no : 65081-1-Ig

Synonyms

B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed death ligand 1



Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65081-1-Ig targets PD-L1 (B7-H1) in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Full length human PD-L1
Full Name CD274 molecule
GenBank accession numberBC069381
Gene symbol CD274
Gene ID (NCBI) 29126
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide, pH 7.2.
Storage ConditionsStore at 2-8°C.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Protocols

Product Specific Protocols
FC protocol for PD-L1 (B7-H1) antibody 65081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols