Anti-Human PD-L1 (B7-H1) (29E.2A3)

PD-L1 (B7-H1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human and More (1)

Applications

FC

Conjugate

Unconjugated

Publications

2

CloneNo.

29E.2A3

Cat no : 65081-1-Ig

Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1

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Validation Data Gallery

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  FC experiment of human PBMCs using 65081-1-Ig

  1X10^6 PHA treated (3d) human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG2b Isotype Control (blue). 1X10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black dashed). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65081-1-Ig

  1X10^6 PHA treated (3d) human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (right), or Mouse IgG2b Isotype Control (left). 1X10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (center). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

65081-1-Ig targets PD-L1 (B7-H1) in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Full length human PD-L1
• Full Name: CD274 molecule
• GenBank Accession Number: BC069381
• Gene Symbol: PD-L1
• Gene ID (NCBI): 29126
• RRID: AB_2918384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publications

Species	Application	Title
human,mouse	WB,IHC	Transl Res; Immune checkpoint activity exacerbate renal interstitial fibrosis progression by enhancing PD-L1 expression in renal tubular epithelial cells; Authors - Yuting Zhang ; KD Validated
mouse		Inflammation; Protective Effects of a Dihydrodiazepine Against Endotoxin Shock Through Suppression of TLR4/NF-κB/IRF3 Signaling Pathways; Authors - Hamza Hanieh