CoraLite®647 Anti-Human PD-L1 (B7-H1) (29E.2A3)

PD-L1 (B7-H1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

29E.2A3

Cat no : CL647-65081

Synonyms

B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed death ligand 1



Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65081-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65081 targets PD-L1 (B7-H1) in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Full length human PD-L1
Full Name CD274 molecule
GenBank accession numberBC069381
Gene symbol CD274
Gene ID (NCBI) 29126
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Protocols

Product Specific Protocols
FC protocol for CL647 PD-L1 (B7-H1) antibody CL647-65081Download protocol
Standard Protocols
Click here to view our Standard Protocols