Anti-Mouse PD-L1/CD274 (10F.9G2) Rat IgG2a Recombinant Antibody, PBS Only

• 

  FC experiment of mouse splenocytes using 65685-2-TR (same clone as 65685-2-PBS)

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Rat IgG2a RecAb (65685-1-TR, Clone: 10F.9G2) and PE anti-rat IgG2a Antibody. Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 65685-2-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 65685-2-TR (same clone as 65685-2-PBS)

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Rat IgG2a RecAb (65685-1-TR, Clone: 10F.9G2) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and PE anti-rat IgG2a Antibody. 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Rat IgG2a RecAb (65685-1-TR, Clone: 10F.9G2) (black, dashed) and PE anti-rat IgG2a Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65685-2-PBS in a different storage buffer formulation.

PD-L1/CD274 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65685-2-PBS

Clone No.10F.9G2

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

Cd274, PD-L1, B7 H1, PD L1, Pdcd1l1

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Product Information

65685-2-PBS targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: CD274 antigen
• GenBank Accession Number: BC066841
• Gene Symbol: PD-L1
• Gene ID (NCBI): 60533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9EP73
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.