PDCD1LG2/PD-L2 Polyclonal antibody

PDCD1LG2/PD-L2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27406-1-AP

Synonyms

B7 DC, B7DC, Btdc, Butyrophilin B7 DC, CD273, PD 1 ligand 2, PD L2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, Programmed death ligand 2

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Validation Data Gallery

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  WB analysis using 27406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27406-1-AP (PDCD1LG2/PD-L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Raji cells, mouse heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27406-1-AP targets PDCD1LG2/PD-L2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDCD1LG2/PD-L2 fusion protein Ag26384
• Full Name: programmed cell death 1 ligand 2
• Calculated Molecular Weight: 273 aa, 31 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC074766
• Gene Symbol: PDCD1LG2
• Gene ID (NCBI): 80380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Programmed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).