PDCD4 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84162-3-RR (same clone as 84162-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84162-3-RR (PDCD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84162-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84162-3-RR (same clone as 84162-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDCD4 Recombinant antibody (84162-3-RR, Clone:241463E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84162-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01098-1

  Cytometric bead array standard curve of MP01098-1, PDCD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84162-3-PBS. Detection antibody: 84162-1-PBS. Standard: Ag3288. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01098-2

  Cytometric bead array standard curve of MP01098-2, PDCD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84162-3-PBS. Detection antibody: 84162-2-PBS. Standard: Ag3288. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84162-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84162-3-PBS against Human PDCD4 was performed. The affinity constant is 75.9 pM.

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  Affinity and Kinetic Characterization of 84162-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84162-3-RR against Human PDCD4 were performed. The affinity constant is 1.54 nM.

PDCD4 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 84162-3-PBS

Clone No.241463E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

Tis, PDCD 4, Nuclear antigen H731-like, H731, Dug

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Product Information

84162-3-PBS targets PDCD4 as part of a matched antibody pair:

MP01098-1: 84162-3-PBS capture and 84162-1-PBS detection (validated in Cytometric bead array)

MP01098-2: 84162-3-PBS capture and 84162-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDCD4 fusion protein Ag3288
• Full Name: programmed cell death 4 (neoplastic transformation inhibitor)
• Calculated Molecular Weight: 469 aa, 52 kDa
• Observed Molecular Weight: 54-64 kDa
• GenBank Accession Number: BC026104
• Gene Symbol: PDCD4
• Gene ID (NCBI): 27250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q53EL6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.