PDCD6/ALG2 Recombinant antibody

• 

  WB analysis using 85792-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85792-5-RR (PDCD6/ALG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 85792-5-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85792-5-RR (PDCD6/ALG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PDCD6/ALG2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85792-5-RR

Clone No.250087B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ALG2, PDCD6, ALG 2, ALG-2, Apoptosis-linked gene 2 protein homolog

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells, K-562 cells, SK-BR-3 cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85792-5-RR targets PDCD6/ALG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDCD6/ALG2 fusion protein Ag2949
• Full Name: programmed cell death 6
• Calculated Molecular Weight: 191 aa, 22 kDa
• Observed Molecular Weight: 15-22 kDa
• GenBank Accession Number: BC012384
• Gene Symbol: PDCD6
• Gene ID (NCBI): 10016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75340
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Programmed cell death protein 6 (PDCD6) is also named as ALG2. PDCD6 is an evolutionarily conserved Ca2+-binding protein. PDCD6 is required for coat protein complex II-dependent endoplasmic reticulum-to-Golgi apparatus vesicular transport in the cytoplasm (PMID: 39934130). PDCD6 is involved in regulating multifaceted and pleiotropic cellular processes in different cellular compartments. The nuclear PDCD6 regulates apoptosis and alternative splicing. And cytoplasmic PDCD6 is involved in the regulation of cytoskeletal dynamics and innate immune responses. Additionally, membranous PDCD6 participates in membrane repair through endosomal sorting complex required for transport complex-dependent membrane budding. (PMID: 38436472). PDCD6 also acts as a regulator of cytosolic DNA-induced immune responses by modulating STING transport (PMID: 34787301).