PDCD6/ALG2 Polyclonal antibody

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  WB analysis using 12303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12303-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 12303-1-AP

  IP result of anti-PDCD6 (IP:12303-1-AP, 3ug; Detection:12303-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human lung cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

PDCD6/ALG2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12303-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ALG2, PDCD6, PEF1B, Apoptosis-linked gene 2 protein homolog, ALG-2

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Product Information

12303-1-AP targets PDCD6/ALG2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDCD6/ALG2 fusion protein Ag2949
• Full Name: programmed cell death 6
• Calculated Molecular Weight: 191 aa, 22 kDa
• Observed Molecular Weight: 15-22 kDa
• GenBank Accession Number: BC012384
• Gene Symbol: PDCD6
• Gene ID (NCBI): 10016
• RRID: AB_2162459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75340
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; Structural basis for dimerization quality control.; Authors - Elijah L Mena
human	IF	Science; Triggered recruitment of ESCRT machinery promotes endolysosomal repair.; Authors - Michael L Skowyra
human	WB,IF	Cell; Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.; Authors - Colleen A McGourty ; KD Validated
human	WB	Mol Cell; Co-adaptor driven assembly of a CUL3 E3 ligase complex.; Authors - David Akopian
human	WB,IF	Cell Rep; Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.; Authors - Johannes Westman ; KD Validated
human	IF	J Cell Biol; Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters; Authors - Femmy C Stempels