PDE3B Polyclonal antibody

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  WB analysis of HL-60 using 25290-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25290-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25290-1-AP

  IP result of anti-PDE3B (IP:25290-1-AP, 5ug; Detection:25290-1-AP 1:300) with HeLa cells lysate 800ug.

PDE3B Polyclonal Antibody for WB, IP, ELISA

Cat No. 25290-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Synonyms

CGIP1, CGI-PDE B, cGIPDE1, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, Cyclic GMP-inhibited phosphodiesterase B

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25290-1-AP targets PDE3B in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE3B fusion protein Ag18064
• Full Name: phosphodiesterase 3B, cGMP-inhibited
• Calculated Molecular Weight: 1112 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC136565
• Gene Symbol: PDE3B
• Gene ID (NCBI): 5140
• RRID: AB_2880010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13370
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE3B is a cyclic nucleotide phosphodiesterase with dual specificity for cAMP and cGMP. It plays crucial roles in regulating physiological processes such as angiogenesis and cardiac contractility. PDE3B can be activated by insulin and is involved in the antilipolytic actions of insulin. Additionally, PDE3B forms macromolecular complexes that are important for its activation and interaction with other proteins (PMID: 22001403, PMID: 26031333).

Publications

Species	Application	Title
mouse,human	WB	Int J Mol Sci; Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions; Authors - Ning Liu