Tested Applications
Positive WB detected in | HeLa cells, mouse adipose tissue, Jurkat cells |
Positive IHC detected in | mouse adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85996-1-RR targets PDE3B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PDE3B fusion protein Ag19190 Predict reactive species |
Full Name | phosphodiesterase 3B, cGMP-inhibited |
Calculated Molecular Weight | 1112 aa, 124 kDa |
Observed Molecular Weight | 124 kDa |
GenBank Accession Number | BC136565 |
Gene Symbol | PDE3B |
Gene ID (NCBI) | 5140 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13370 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDE3B is a cyclic nucleotide phosphodiesterase with dual specificity for cAMP and cGMP. It plays crucial roles in regulating physiological processes such as angiogenesis and cardiac contractility. PDE3B can be activated by insulin and is involved in the antilipolytic actions of insulin. Additionally, PDE3B forms macromolecular complexes that are important for its activation and interaction with other proteins (PMID: 22001403, PMID: 26031333).
Protocols
Product Specific Protocols | |
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WB protocol for PDE3B antibody 85996-1-RR | Download protocol |
IHC protocol for PDE3B antibody 85996-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |