Tested Applications
| Positive WB detected in | human brain tissue, A2780 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16226-1-AP targets PDE4A in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8018 Product name: Recombinant human PDE4A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 297-647 aa of BC019864 Sequence: MVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGHPPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQREHQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT Predict reactive species |
| Full Name | phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila) |
| Calculated Molecular Weight | 98 kDa |
| Observed Molecular Weight | 125-130 kDa |
| GenBank Accession Number | BC019864 |
| Gene Symbol | PDE4A |
| Gene ID (NCBI) | 5141 |
| RRID | AB_2252090 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27815 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDE4A antibody 16226-1-AP | Download protocol |
| WB protocol for PDE4A antibody 16226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDE4A Polyclonal antibody (Cat# 16226-1-AP) has 13 citations across 11 journals, including Acta Pharmacol Sin, Br J Pharmacol, Biochem Pharmacol, Int J Mol Sci, Int Immunopharmacol, Int J Neuropsychopharmacol, Cell Signal, Am J Physiol Renal Physiol, Eur Rev Med Pharmacol Sci, Neuroscience, and Drugs R D. Research fields span neuroinflammation, cAMP/PKA signaling, PDE4 inhibition, alcohol addiction, PTSD, depression, Alzheimer's disease, cardiac development, vascular tone, renal injury, psoriasis, and atopic dermatitis.
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin PDE4 inhibitor rolipram dynamically regulates the balance between D1-MSNs and D2-MSNs in the DMS to modulate abnormal "Go" behavior associated with alcohol addiction. | ||
Br J Pharmacol Involvement of 3',5'-cyclic inosine monophosphate in cystathionine γ-lyase-dependent regulation of the vascular tone. | ||
Biochem Pharmacol DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling. | ||
Int Immunopharmacol Inhibition of phosphodiesterase-4 by rolipram alleviates anxiety-like behavior in mice with PTSD by modulating neuroinflammation and synaptic plasticity via cAMP signaling | ||
Int J Neuropsychopharmacol Upregulation of Phosphodiesterase 7A Contributes to Concurrent Pain and Depression via Inhibition of cAMP-PKA-CREB-BDNF Signaling and Neuroinflammation in the Hippocampus of Mice |
