PDE4A Polyclonal antibody

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• KD/KO Validated

PDE4A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 16226-1-AP

Synonyms

DPDE2, PDE4, PDE46, PDE4A

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Validation Data Gallery

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  WB analysis of HeLa using 16226-1-AP

  WB result of PDE4A antibody (16226-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDE4A transfected HeLa cells.

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  WB analysis of human brain using 16226-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16226-1-AP (PDE4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16226-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16226-1-AP (PDE4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16226-1-AP

  IP result of anti-PDE4A (IP:16226-1-AP, 4ug; Detection:16226-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 16226-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16226-1-AP (PDE4A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16226-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16226-1-AP (PDE4A antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below

Product Information

16226-1-AP targets PDE4A in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE4A fusion protein Ag8018
• Full Name: phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila)
• Calculated Molecular Weight: 98 kDa
• Observed Molecular Weight: 125-130 kDa
• GenBank Accession Number: BC019864
• Gene Symbol: PDE4A
• Gene ID (NCBI): 5141
• RRID: AB_2252090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Br J Pharmacol; Involvement of 3',5'-cyclic inosine monophosphate in cystathionine γ-lyase-dependent regulation of the vascular tone.; Authors - Emma Mitidieri
mouse	WB	Int J Mol Sci; Phosphodiesterases Expression during Murine Cardiac Development.; Authors - Thays Maria da Conceição Silva Carvalho
mouse	WB	Biochem Pharmacol; DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling.; Authors - Heng Li
human	WB,IHC	Cell Signal; Phosphodiesterase 4 in inflammatory diseases: Effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex.; Authors - Peter H Schafer
mouse	WB	Cell Signal; RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.; Authors - Menbere Wendimu
human	IHC	Drugs R D; Apremilast Normalizes Gene Expression of Inflammatory Mediators in Human Keratinocytes and Reduces Antigen-Induced Atopic Dermatitis in Mice.; Authors - Peter H Schafer