Tested Applications
| Positive WB detected in | K-562 cells, mouse brain tissue, mouse pancreas tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12918-1-AP targets PDE4D in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3588 Product name: Recombinant human PDE4D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 170-518 aa of BC036319 Sequence: YHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVFNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT Predict reactive species |
| Full Name | phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) |
| Calculated Molecular Weight | 809 aa, 91 kDa |
| Observed Molecular Weight | 91-110 kDa, 66-84 kDa |
| GenBank Accession Number | BC036319 |
| Gene Symbol | PDE4D |
| Gene ID (NCBI) | 5144 |
| RRID | AB_2161097 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08499 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer disease(PMID:17353396).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDE4D antibody 12918-1-AP | Download protocol |
| IHC protocol for PDE4D antibody 12918-1-AP | Download protocol |
| WB protocol for PDE4D antibody 12918-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDE4D antibody (Cat# 12918-1-AP) has 34 citations published in journals such as Cell Metabolism, Cancer Letters, Cell Reports, JCI Insight, FASEB Journal, Acta Pharmaceutica Sinica B, and Journal of Ethnopharmacology. Research fields include oncology (HCC, melanoma, pancreatic and breast cancer), metabolic disorders (NAFLD, diabetes, obesity), inflammation (ulcerative colitis, atopic dermatitis), cardiovascular disease, neurology, and pharmacology. Applications are primarily WB, IF, IHC, and Co-IP.
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Targeting PDE4 as a promising therapeutic strategy in chronic ulcerative colitis through modulating mucosal homeostasis. | ||
Adv Sci (Weinh) Cinobufagin Directly Targets PDE4D to Disrupt Fibroblast-Dendritic Cell Crosstalk in Atopic Dermatitis. | ||
Pharmacol Res Overexpression of PDE4D in mouse liver is sufficient to trigger NAFLD and hypertension in a CD36-TGF-β1 pathway: therapeutic role of roflumilast | ||
Cancer Lett PDE4D binds and interacts with YAP to cooperatively promote HCC progression.
| ||
Cancer Lett PDE4D/cAMP/IL-23 axis determines the immunotherapy efficacy of lung adenocarcinoma via activating the IL-9 autocrine loop of cytotoxic T lymphocytes |
Reviews
What customers say
The single review for this product rates it 5 stars, describing it as "great and specific." The reviewer used it for Western Blot at a 1:500 dilution on protein from fibroblasts, confirming strong specificity and reliable performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
RICHA (Verified Customer) (10-09-2019) | Great and specific
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