PDE4D Polyclonal antibody

• Featured Product
• KD/KO Validated

PDE4D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (1)

Cat no : 12918-1-AP

Synonyms

PDE43, EC:3.1.4.53, DPDE3, cAMP-specific phosphodiesterase 4D, 3',5'-cyclic-AMP phosphodiesterase 4D

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Validation Data Gallery

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  WB analysis using 12918-1-AP

  K562 cell lysate were subjected to SDS PAGE followed by western blot with 12918-1-AP (PDE4D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12918-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12918-1-AP (PDE4D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12918-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12918-1-AP (PDE4D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse colon using 12918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12918-1-AP (PDE4D Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse colon using 12918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12918-1-AP (PDE4D Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 12918-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PDE4D antibody (12918-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse brain tissue, mouse pancreas tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

12918-1-AP targets PDE4D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE4D fusion protein Ag3588
• Full Name: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Calculated Molecular Weight: 809 aa, 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC036319
• Gene Symbol: PDE4D
• Gene ID (NCBI): 5144
• RRID: AB_2161097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer disease(PMID:17353396).

Publications

Species	Application	Title
mouse	WB	Cell Metab; FGF1 and insulin control lipolysis by convergent pathways.; Authors - Gencer Sancar
human	WB	Acta Pharm Sin B; Targeting PDE4 as a promising therapeutic strategy in chronic ulcerative colitis through modulating mucosal homeostasis.; Authors - Heng Li
mouse	WB	Cell Rep; miR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes.; Authors - Keita Tsujimura
human	WB	JCI Insight; Inhibition of phosphodiesterase 4D suppresses mTORC1 signaling and pancreatic cancer growth; Authors - Mi-Hyeon Jeong
	WB	Diabetes; Salt inducible kinase 1 terminates cAMP signaling by an evolutionarily conserved negative feedback loop in beta cells.; Authors - Min-Jung Kim
human	WB,IHC,IF	Cancer Lett; PDE4D binds and interacts with YAP to cooperatively promote HCC progression.; Authors - Huili Ren ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

RICHA (Verified Customer) (10-09-2019)

Great and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from fibroblasts

PDE4D Antibody Western Blot, validation (1:500 dilution) in Protein from fibroblasts (Cat no:12918-1-AP)