PDE4D Monoclonal antibody

PDE4D Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C4B9

Reviews (1)

Cat no : 67062-1-Ig

Synonyms

PDE43, EC:3.1.4.53, DPDE3, cAMP-specific phosphodiesterase 4D, 3',5'-cyclic-AMP phosphodiesterase 4D

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Validation Data Gallery

• 

  WB analysis using 67062-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 67062-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67062-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 67062-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67062-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 67062-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDE4D antibody (67062-1-Ig, Clone: 1C4B9 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: A549 cells, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, pig brain tissue, mouse cerebellum tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67062-1-Ig targets PDE4D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDE4D fusion protein Ag8302
• Full Name: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Calculated Molecular Weight: 809 aa, 91 kDa
• Observed Molecular Weight: 91-110 kDa, 66-84 kDa
• GenBank Accession Number: BC036319
• Gene Symbol: PDE4D
• Gene ID (NCBI): 5144
• RRID: AB_2882372
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also known as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram (PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in the hippocampus from a patient with advanced Alzheimer disease (PMID:17353396). Bands of 90-110 kDa correspond to the long PDE4D isoforms, and bands of 66-84 kDa are the short PDE4D isoforms (PMID: 17215491).

Publications

Species	Application	Title
human	WB	Cancer Med; Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma; Authors - Yimin Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (06-06-2023)

Protein lysates from A549 cells were subjected to SDS-PAGE followed by immunoblotting with 67062-1-Ig (mouse anti-PDE4D antibody) at dilution of 1:10 000 incubated overnight at 4°C. Secondary antibody: Donkey-anti-mouse (Alexa Fluor 680) at 1:20000 dilution incubated at room temperature for 1 hour.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10 000
• Cell Tissue Type: Human lung cancer cell A549

PDE4D Antibody Western Blot validation (1:10 000 dilution) in Human lung cancer cell A549 (Cat no:67062-1-Ig)