PDE4D Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of mouse pancreas using 86745-1-RR (same clone as 86745-1-PBS)

  Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 86745-1-RR (PDE4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86745-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 86745-1-RR (same clone as 86745-1-PBS)

  Rat brain tissue were subjected to SDS PAGE followed by western blot with 86745-1-RR (PDE4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86745-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 86745-1-RR (same clone as 86745-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 86745-1-RR (PDE4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86745-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86745-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86745-1-RR against Human PDE4D were performed. The affinity constant is 3.51 nM.

PDE4D Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86745-1-PBS

Clone No.251427D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

3',5'-cyclic-AMP phosphodiesterase 4D, cAMP-specific phosphodiesterase 4D, DPDE3, EC:3.1.4.53, PDE43

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Product Information

86745-1-PBS targets PDE4D in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3588

  Product name: Recombinant human PDE4D protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 170-518 aa of BC036319

  Sequence: YHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVFNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT

• Full Name: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Calculated Molecular Weight: 809 aa, 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC036319
• Gene Symbol: PDE4D
• Gene ID (NCBI): 5144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q08499
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDE4D (cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43, and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has some isoforms, and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer's disease(PMID:17353396).