PDE8A Polyclonal antibody, PBS Only

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• PDE8A Polyclonal antibody, PBS Only (13956-1-PBS)

• 

  WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 13956-1-AP (same clone as 13956-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 13956-1-AP (same clone as 13956-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

  IP result of anti-PDE8A (IP:13956-1-AP, 5ug; Detection:13956-1-AP 1:1000) with mouse testis tissue lysate 8000ug. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

PDE8A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 13956-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

13956-1-PBS targets PDE8A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5069

  Product name: Recombinant human PDE8A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 530-829 aa of BC060762

  Sequence: FLHCSESTLRSWLQIIEANYHSSNPYHNSTHSADVLHATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVNSINKPLATLEENGETDKNQEVINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEWAARISEEYFSQTDEEKQQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE

• Full Name: phosphodiesterase 8A
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 93 kDa, 88 kDa
• GenBank Accession Number: BC060762
• Gene Symbol: PDE8A
• Gene ID (NCBI): 5151
• RRID: AB_2161728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60658
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDE8A(high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A) belongs to the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during β-adrenergic receptor (βAR) activation in ventricular myocytes(PMID:20353794). The full length protein contains several sites for protein phosphorylation, N-glycosylation, N-myristoylation, and amidation. It has 5 isoforms produced by alternative splicing with the molecular mass of 93 kDa, 88 kDa, 51 kDa and the isoform 4 and 5 have the same molecular mass of 66 kDa.(PMID:11738832).