Tested Applications
| Positive WB detected in | NIH/3T3 cells, C2C12 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84388-5-RR targets PDGFR beta/CD140b in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1533 Product name: Recombinant Mouse PDGFR beta protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 32-531 aa of NM_008809.2 Sequence: LVITPPGPEFVLNISSTFVLTCSGSAPVMWEQMSQVPWQEAAMNQDGTFSSVLTLTNVTGGDTGEYFCVYNNSLGPELSERKRIYIFVPDPTMGFLPMDSEDLFIFVTDVTETTIPCRVTDPQLEVTLHEKKVDIPLHVPYDHQRGFTGTFEDKTYICKTTIGDREVDSDTYYVYSLQVSSINVSVNAVQTVVRQGESITIRCIVMGNDVVNFQWTYPRMKSGRLVEPVTDYLFGVPSRIGSILHIPTAELSDSGTYTCNVSVSVNDHGDEKAINISVIENGYVRLLETLGDVEIAELHRSRTLRVVFEAYPMPSVLWLKDNRTLGDSGAGELVLSTRNMSETRYVSELILVRVKVSEAGYYTMRAFHEDDEVQLSFKLQVNVPVRVLELSESHPANGEQTIRCRGRGMPQPNVTWSTCRDLKRCPRKLSPTPLGNSSKEESQLETNVTFWEEDQEYEVVSTLRLRHVDQPLSVRCMLQNSMGGDSQEVTVVPHSLPFKV Predict reactive species |
| Full Name | platelet derived growth factor receptor, beta polypeptide |
| Calculated Molecular Weight | 123kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | NM_008809.2 |
| Gene Symbol | PDGFR beta |
| Gene ID (NCBI) | 18596 |
| RRID | AB_3671924 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05622-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Platelet-derived growth Factor Receptor Beta (PDGFRβ) is a member of the receptor tyrosine kinase family of proteins, which plays a crucial role in various physiological and pathological conditions.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDGFR beta/CD140b antibody 84388-5-RR | Download protocol |
| WB protocol for PDGFR beta/CD140b antibody 84388-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One reviewer tested this antibody by immunohistochemistry on mouse kidney, placenta, and colon tissues as an alternative to αSMA for identifying pericytes in murine tumor tissues. Despite optimizing multiple conditions—including varying antigen retrieval times (32–40 min), dilutions (1:50 and 1:100), and incubation durations (40 min to overnight)—no specific staining was detected in any of the tested tissues. The reviewer concluded the antibody did not produce detectable labeling under their experimental setup.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ana (Verified Customer) (03-14-2026) | We tested this antibody as an alternative to αSMA for the identification of pericytes by immunohistochemistry in murine tumor tissues with strong angiogenesis. To optimize staining, we performed several conditions. Initial testing was done in colon tissue (32 min antigen retrieval, 1:100 dilution, 40 min incubation). We then tested placenta and testis with 40 min antigen retrieval and 1:100 dilution. Additional experiments were performed using 40 min antigen retrieval with a higher concentration (1:50) in kidney, placenta, and colon. Finally, we tested 40 min antigen retrieval with 1:50 dilution and overnight incubation in kidney, placenta, and colon. Despite testing multiple antigen retrieval conditions, antibody concentrations, and incubation times, we were unable to detect specific staining in any of the tested tissues. Under our experimental conditions, the antibody did not produce detectable labeling by immunohistochemistry, at least in our hands.
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