CoraLite® Plus 647 Anti-Mouse PDGFR alpha/CD140a Rabbit Recombinant Antibody

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  FC experiment of NIH/3T3 using CL647-98256

  1x10^6 NIH/3T3 cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse PDGFR alpha/CD140a Rabbit RecAb (CL647-98256, Clone: 242025B3) (red) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

PDGFR alpha/CD140a Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98256

Clone No.242025B3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

242025B3, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a

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Tested Applications

• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98256 targets PDGFR alpha/CD140a in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDGFR alpha/CD140a fusion protein Eg1625
• Full Name: platelet derived growth factor receptor, alpha polypeptide
• Calculated Molecular Weight: 123kDa
• GenBank Accession Number: NM_001083316.2
• Gene Symbol: Pdgfra
• Gene ID (NCBI): 18595
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26618-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Platelet-derived growth factor receptor alpha (PDGFR alpha, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.