|Positive WB detected in||COLO 320 cells, rat heart tissue, mouse thymus tissue, rat thymus tissue, HEK-293 cell, HepG2 cells, mouse heart tissue, rat brain tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HEK-293T cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11245-1-AP targets PDI in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PDI fusion protein Ag1747|
|Full Name||prolyl 4-hydroxylase, beta polypeptide|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||57 kDa|
|GenBank accession number||BC014504|
|Gene ID (NCBI)||5034|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).
|Product Specific Protocols|
|WB protocol for PDI antibody 11245-1-AP||Download protocol|
|IHC protocol for PDI antibody 11245-1-AP||Download protocol|
|IF protocol for PDI antibody 11245-1-AP||Download protocol|
|IP protocol for PDI antibody 11245-1-AP||Download protocol|
|FC protocol for PDI antibody 11245-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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