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PDI Polyclonal antibody

PDI Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11245-1-AP

Synonyms

DSI, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase



Tested Applications

Positive WB detected inCOLO 320 cells, rat heart tissue, mouse thymus tissue, rat thymus tissue, HEK-293 cell, HepG2 cells, mouse heart tissue, rat brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11245-1-AP targets PDI in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDI fusion protein Ag1747
Full Name prolyl 4-hydroxylase, beta polypeptide
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC014504
Gene symbol P4HB
Gene ID (NCBI) 5034
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Protocols

Product Specific Protocols
WB protocol for PDI antibody 11245-1-APDownload protocol
IHC protocol for PDI antibody 11245-1-APDownload protocol
IF protocol for PDI antibody 11245-1-APDownload protocol
IP protocol for PDI antibody 11245-1-APDownload protocol
FC protocol for PDI antibody 11245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Mol Cell Endocrinol

Defective endoplasmic reticulum export causes proinsulin misfolding in pancreatic β cells.

Authors - Ruimin Zhu

Virology

NSP2 forms viroplasms during Dendrolimus punctatus cypovirus infection.

Authors - Congrui Xu
IHC

JCI Insight

Disease-modifying effects of orally bioavailable NF-κB inhibitors in dystrophin-deficient muscle.

Authors - David W Hammers
mouseIF

Biochim Biophys Acta

Halofuginone improves muscle-cell survival in muscular dystrophies.

Authors - Anna Bodanovsky
humanWB

Cell Death Dis

Mutations in the coat complex II component SEC23B promote colorectal cancer metastasis.

Authors - Chunyuan Yang
humanWB

Oncotarget

PMID: 28402965

Authors - Yan-Feng Huang