PDI Polyclonal antibody

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This PDI antibody (11245-1-AP) is a rabbit polyclonal antibody that targets PDI in WB, IHC, IF/ICC, FC (Intra), IP, ELISA and is suitable for human, mouse, rat, zebrafish samples. The 11245-1-AP antibody has 84 publication citations.

Cat No. 11245-1-AP Copied!

Publications (84)

Reviews (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

P4HB, Cellular thyroid hormone-binding protein, CLCRP1, DSI, EC:5.3.4.1

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  WB analysis using 11245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11245-1-AP (PDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis using 11245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11245-1-AP (PDI antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 11245-1-AP

  WB result of PDI antibody (11245-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HepG2 using 11245-1-AP

  IP result of anti-PDI (IP:11245-1-AP, 4ug; Detection:11245-1-AP 1:2000) with HepG2 cells lysate 2600 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human prostate cancer using 11245-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11245-1-AP (PDI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse liver using 11245-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11245-1-AP (PDI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human colon using 11245-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11245-1-AP (PDI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IF Staining of HepG2 using 11245-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDI antibody (11245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 11245-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (11245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 11245-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PDI (11245-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells, HepG2 cells, mouse heart tissue, rat brain tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human prostate cancer tissue, human colon tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11245-1-AP targets PDI in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, rat, pig, monkey, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1747

  Product name: Recombinant human PDI protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 324-508 aa of NM_000918

  Sequence: MTKYKPESEELTAEWITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL

• Full Name: prolyl 4-hydroxylase, beta polypeptide
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: NM_000918
• Gene Symbol: PDI
• Gene ID (NCBI): 5034
• RRID: AB_2298937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07237
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Publications

What published studies show

The PDI Polyclonal antibody (Cat# 11245-1-AP), targeting PDIA1/P4HB, has accumulated 84 citations published in journals including Nature, Nature Methods, Nature Communications, Autophagy, Cell Death & Differentiation, Cell Reports, Journal of Clinical Investigation, Oncogenesis, and eLife. Associated research fields span ER stress, protein disulfide bond folding, autophagy, ferroptosis, cancer biology (breast, ovarian, pancreatic, glioblastoma), cardiovascular disease, neurodegeneration, lipid metabolism, viral infections, and diabetes.

Automatically generated from the 84 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	IHC	Nature; Heart repair by reprogramming non-myocytes with cardiac transcription factors.; Authors - Song Kunhua K
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
mouse	WB	Nat Methods; Global analysis of endogenous protein disorder in cells; Authors - Shouxiang Zhang
human	WB,IHC	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
human	WB	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni
	WB	Nat Commun; Gonococcal OMV-delivered PorB induces epithelial cell mitophagy; Authors - Shuai Gao

Reviews

5.0 out of 5

2 verified customer reviews

What customers say

Both reviews rated the product 5 stars for Western Blot. One user reported it worked well at a 1:2000 dilution on MSCs, while another used it at 1:500 on zebrafish embryos. Overall, customers found the antibody reliable and effective for WB applications across different sample types.

Automatically generated from the 2 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Udesh (Verified Customer) (08-19-2024)

Worked well at 1:2000

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MSCs

PDI Antibody Western Blot validation (1:2000 dilution) in MSCs (Cat no:11245-1-AP)

Anastasia (Verified Customer) (08-07-2024)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Zebrafish embryos

PDI Antibody Western Blot validation (1:500 dilution) in Zebrafish embryos (Cat no:11245-1-AP)