PDIA6 Monoclonal antibody, PBS Only

PDIA6 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G2D4

Cat no : 66669-1-PBS

Synonyms

ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7

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Validation Data Gallery

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  WB analysis using 66669-1-Ig (same clone as 66669-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66669-1-Ig (PDIA6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66669-1-Ig (PDIA6 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66669-1-PBS targets PDIA6 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDIA6 fusion protein Ag27608
• Full Name: protein disulfide isomerase family A, member 6
• Calculated Molecular Weight: 48 kDa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC001312
• Gene Symbol: PDIA6
• Gene ID (NCBI): 10130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PDIA6 (Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze the formation, reduction, and isomerization of disulfide bonds in proteins and is thought to play a role in the folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.