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  • KD/KO Validated

PDLIM1/CLP36 Polyclonal antibody

PDLIM1/CLP36 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11674-1-AP

Synonyms

CLIM1, CLP 36, CLP36, Elfin, hCLIM1, LIM domain protein CLP 36, PDLIM1, PDZ and LIM domain 1, PDZ and LIM domain protein 1



Tested Applications

Positive WB detected inmouse lung tissue, human ileum tissue, human lung tissue, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11674-1-AP targets PDLIM1/CLP36 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDLIM1/CLP36 fusion protein Ag2274
Full Name PDZ and LIM domain 1
Calculated molecular weight 329 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000915
Gene symbol PDLIM1
Gene ID (NCBI) 9124
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)

Protocols

Product Specific Protocols
WB protocol for PDLIM1/CLP36 antibody 11674-1-APDownload protocol
IHC protocol for PDLIM1/CLP36 antibody 11674-1-APDownload protocol
IF protocol for PDLIM1/CLP36 antibody 11674-1-APDownload protocol
IP protocol for PDLIM1/CLP36 antibody 11674-1-APDownload protocol
FC protocol for PDLIM1/CLP36 antibody 11674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB,IF

Neurosci Lett

CLP36 interacts with palladin in dorsal root ganglion neurons.

Authors - Hasegawa Tomohiko T
humanWB,IF

Exp Cell Res

CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts.

Authors - Miyazaki Kazufumi K
  • KD Validated
mouseWB

Open Biol

Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36.

Authors - Shiguo Li
humanWB

EMBO Rep

Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB.

Authors - Yusha Wang
mouseWB

Autophagy

Autophagy regulates spermatid differentiation via degradation of PDLIM1.

Authors - Yongliang Shang
mouseWB,IF

Autophagy

Autophagy is required for ectoplasmic specialization assembly in Sertoli cells.

Authors - Chao Liu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-03-2019)

PDLIM1 shows up as a clear band at the correct molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
PDLIM1/CLP36 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:11674-1-AP)