- Featured Product
- KD/KO Validated
PDLIM1/CLP36 Polyclonal antibody
PDLIM1/CLP36 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 11674-1-AP
|Positive WB detected in||mouse lung tissue, human ileum tissue, human lung tissue, mouse heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11674-1-AP targets PDLIM1/CLP36 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PDLIM1/CLP36 fusion protein Ag2274|
|Full Name||PDZ and LIM domain 1|
|Calculated molecular weight||329 aa, 36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC000915|
|Gene ID (NCBI)||9124|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)
|Product Specific Protocols|
|WB protocol for PDLIM1/CLP36 antibody 11674-1-AP||Download protocol|
|IHC protocol for PDLIM1/CLP36 antibody 11674-1-AP||Download protocol|
|IF protocol for PDLIM1/CLP36 antibody 11674-1-AP||Download protocol|
|IP protocol for PDLIM1/CLP36 antibody 11674-1-AP||Download protocol|
|FC protocol for PDLIM1/CLP36 antibody 11674-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CLP36 interacts with palladin in dorsal root ganglion neurons.
Exp Cell Res
CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts.
Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36.
Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB.
Autophagy regulates spermatid differentiation via degradation of PDLIM1.
Autophagy is required for ectoplasmic specialization assembly in Sertoli cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-03-2019)
PDLIM1 shows up as a clear band at the correct molecular weight.