PDLIM1/CLP36 Recombinant antibody

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  WB analysis using 85027-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85027-5-RR (PDLIM1,CLP36 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85027-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDLIM1/CLP36 antibody (85027-5-RR, Clone: 242585C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 85027-5-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PDLIM1/CLP36 Recombinant antibody (85027-5-RR, Clone:242585C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85027-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85027-5-RR against Human PDLIM1/CLP36 were performed. The affinity constant is 6.23 nM.

PDLIM1/CLP36 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 85027-5-RR

Clone No.242585C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

PDLIM1, 242585C7, CLIM1, CLP36, CLP-36

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Tested Applications

• Positive WB detected in: C2C12 cells, Caco-2 cells, HEK-293T cells, Saos-2 cells, rat lung tissue, human heart tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85027-5-RR targets PDLIM1/CLP36 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDLIM1/CLP36 fusion protein Ag2274
• Full Name: PDZ and LIM domain 1
• Calculated Molecular Weight: 329 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000915
• Gene Symbol: PDLIM1/CLP36
• Gene ID (NCBI): 9124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00151
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)