Various lysates were subjected to SDS PAGE followed by western blot with 85027-5-RR (PDLIM1,CLP36 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 85027-5-RR (PDLIM1,CLP36 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 85027-5-RR
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85027-5-RR (PDLIM1/CLP36 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85027-5-RR (PDLIM1/CLP36 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 85027-5-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDLIM1/CLP36 antibody (85027-5-RR, Clone: 242585C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDLIM1/CLP36 antibody (85027-5-RR, Clone: 242585C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HepG2 using 85027-5-RR
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PDLIM1/CLP36 Recombinant antibody (85027-5-RR, Clone:242585C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PDLIM1/CLP36 Recombinant antibody (85027-5-RR, Clone:242585C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 85027-5-RR
Biolayer interferometry (BLl) kinetic assays of 85027-5-RR against Human PDLIM1/CLP36 were performed. The affinity constant is 6.23 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
C2C12 cells, Caco-2 cells, HEK-293T cells, Saos-2 cells, rat lung tissue, human heart tissue
Positive IHC detected in
human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
85027-5-RR targets PDLIM1/CLP36 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)
Various lysates were subjected to SDS PAGE followed by western blot with 85027-5-RR (PDLIM1,CLP36 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human stomach cancer using 85027-5-RR
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85027-5-RR (PDLIM1/CLP36 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 85027-5-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDLIM1/CLP36 antibody (85027-5-RR, Clone: 242585C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC (INTRA) Figures
FC experiment of HepG2 using 85027-5-RR
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PDLIM1/CLP36 Recombinant antibody (85027-5-RR, Clone:242585C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 85027-5-RR
Biolayer interferometry (BLl) kinetic assays of 85027-5-RR against Human PDLIM1/CLP36 were performed. The affinity constant is 6.23 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
PDLIM1/CLP36 Recombinant antibody
Catalog Number
85027-5-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:200-1:800 IF/ICC : 1:250-1:1000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
