PDP2 Polyclonal antibody

PDP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13404-1-AP

Synonyms

KIAA1348, PDP 2, PDP2, PDPC 2

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 13404-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13404-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13404-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 13404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 13404-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 13404-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, HeLa cells, mouse kidney tissue
• Positive IHC detected in: mouse brain tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13404-1-AP targets PDP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDP2 fusion protein Ag4257
• Full Name: pyruvate dehydrogenase phosphatase isoenzyme 2
• Calculated Molecular Weight: 529 aa, 60 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC028030
• Gene Symbol: PDP2
• Gene ID (NCBI): 57546
• RRID: AB_10639505
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDP2, is the Ca2+-insensitive isoform of pyruvate dehydrogenase phosphataseis and found in liver and adipose tissue. In adipose tissue,insulin reduces the concentration dependence of PDP2 activity for Mg2+(PMID:12676647).It catalyzes the dephosphorylation and concomitant reactivation of the mitochondrial pyruvate dehydrogenase multienzyme complex.It can be detected the mature (52 kDa) and the immature (60 kDa) bands in western blot.

Publications

Species	Application	Title
mouse,human	WB	Dev Cell; Rheb mediates neuronal-activity-induced mitochondrial energetics through mTORC1-independent PDH activation.; Authors - Wanchun Yang
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma