PDS5B Recombinant antibody

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  WB analysis using 86088-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86088-2-RR (PDS5B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86088-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86088-2-RR against Human PDS5B were performed. The affinity constant is below 1 pM.

PDS5B Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86088-2-RR

Clone No.250461B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

Androgen-induced proliferation inhibitor, Androgen-induced prostate proliferative shutoff-associated protein AS3, APRIN, AS3, CG008

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86088-2-RR targets PDS5B in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDS5B fusion protein Ag27977
• Full Name: PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
• Calculated Molecular Weight: 165 aa
• Observed Molecular Weight: 166 kDa
• GenBank Accession Number: NM_015032
• Gene Symbol: PDS5B
• Gene ID (NCBI): 23047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NTI5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDS5B (PDS5 Cohesin Scc1-like Protein B) is a protein associated with sister chromatid cohesion and maintenance of chromosome structure. It plays a significant role in cell cycle regulation, DNA repair, and gene expression. PDS5B interacts with the cohesin complex to ensure the proper separation of sister chromatids during cell division. Additionally, PDS5B is crucial in DNA damage repair, particularly during homologous recombination, helping to maintain genomic stability. It also indirectly affects gene expression and chromatin structure by modulating the function of the cohesin complex. PDS5B is widely expressed during embryonic development and is highly expressed in the brain and testes in adulthood. Its dysfunction is associated with multiple diseases, including Cohesinopathies and Cornelia de Lange syndrome.