Validation Data Gallery
|Positive WB detected in||human peripheral blood platelets, HUVEC cells, Jurkat cells, THP-1 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human hepatocirrhosis tissue, human tonsillitis tissue, human liver cancer tissue, human gliomas tissue, human renal cell carcinoma tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:800-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11265-1-AP targets CD31 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD31 fusion protein Ag1787|
|Full Name||platelet/endothelial cell adhesion molecule|
|Calculated molecular weight||83 kDa|
|Observed molecular weight||120-130 kDa|
|GenBank accession number||BC022512|
|Gene ID (NCBI)||5175|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
|Product Specific Protocols|
|WB protocol for CD31 antibody 11265-1-AP||Download protocol|
|IHC protocol for CD31 antibody 11265-1-AP||Download protocol|
|IF protocol for CD31 antibody 11265-1-AP||Download protocol|
|IP protocol for CD31 antibody 11265-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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