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- KD/KO Validated
CD31 Polyclonal antibody
CD31 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Cat no : 11265-1-AP
Validation Data Gallery
|Positive WB detected in
|human peripheral blood platelets, HUVEC cells, Jurkat cells, THP-1 cells
|Positive IP detected in
|Positive IHC detected in
|human liver cancer tissue, human tonsillitis tissue, human placenta tissue, human renal cell carcinoma tissue, human hepatocirrhosis tissue, human kidney tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:2000-1:8000
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11265-1-AP targets CD31 in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human samples.
|human, goat, monkey, rabbit, pig, canine
|Host / Isotype
|Rabbit / IgG
|CD31 fusion protein Ag1787
|platelet/endothelial cell adhesion molecule
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
|Product Specific Protocols
|WB protocol for CD31 antibody 11265-1-AP
|IHC protocol for CD31 antibody 11265-1-AP
|IF protocol for CD31 antibody 11265-1-AP
|IP protocol for CD31 antibody 11265-1-AP
|Click here to view our Standard Protocols
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