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CD31 Polyclonal antibody

CD31 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IP, IHC, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 11265-1-AP

Synonyms

CD31, EndoCAM, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker



Tested Applications

Positive WB detected inhuman peripheral blood platelets, HUVEC cells, Jurkat cells, THP-1 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue, human placenta tissue, human renal cell carcinoma tissue, human hepatocirrhosis tissue, human kidney tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11265-1-AP targets CD31 in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, goat, monkey, rabbit, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD31 fusion protein Ag1787
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC022512
Gene symbol PECAM1
Gene ID (NCBI) 5175
RRIDAB_2299349
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
WB protocol for CD31 antibody 11265-1-APDownload protocol
IHC protocol for CD31 antibody 11265-1-APDownload protocol
IF protocol for CD31 antibody 11265-1-APDownload protocol
IP protocol for CD31 antibody 11265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Bioact Mater

Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration

Authors - Yiting Sun
rabbitWB,IF

Nat Commun

Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction

Authors - Yujie Hua
humanIHC

Sci Transl Med

Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.

Authors - Kana Hasegawa
humanIHC,IF

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
humanIHC

Mol Cancer

CircPTPRA blocks the recognition of RNA N6-methyladenosine through interacting with IGF2BP1 to suppress bladder cancer progression.

Authors - Fei Xie
monkey,humanIHC

Acta Pharm Sin B

Nonclinical safety, tolerance and pharmacodynamics evaluation for meplazumab treating chloroquine-resistant Plasmodium falciparum.

Authors - Kun Zhang