PER1 Polyclonal antibody, PBS Only

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• PER1 Polyclonal antibody, PBS Only (13463-1-PBS)

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  WB analysis using 13463-1-AP (same clone as 13463-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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  WB analysis using 13463-1-AP (same clone as 13463-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13463-1-AP (same clone as 13463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13463-1-AP (same clone as 13463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 13463-1-AP (same clone as 13463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13463-1-AP (same clone as 13463-1-PBS)

  Immunofluorescent analysis of Hela cells, using PER1 antibody 13463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

PER1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 13463-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hPER, hPER1, KIAA0482, PER, period homolog 1 (Drosophila)

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Product Information

13463-1-PBS targets PER1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4249

  Product name: Recombinant human PER1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC028207

  Sequence: MSGPLEGADGGGDPRPGESFCPGGVPSPGPPQHRPCPGPSLADDTDANSNGSSGNESNGHESRGASQRSSHSSSSGNGKDSALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMTALRELKLRLPPERRGKGRSGTLATLQYALACVKQVQANQEYYQQWSLEEGEPCSMDMSTYTLEELEHITSEYTLQNQDTFSVAVSFLTGRIVYISEQAAVLLRCKRDVFRGTRFSELLAPQDVGVFYGSTAPSRLPTWGTGASAGSGLRDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTPYVTKIRVSDGAPAQPCCLLIAE

• Full Name: period homolog 1 (Drosophila)
• Calculated Molecular Weight: 1290 aa, 136 kDa
• Observed Molecular Weight: 110 kDa, 50-55 kDa
• GenBank Accession Number: BC028207
• Gene Symbol: PER1
• Gene ID (NCBI): 5187
• RRID: AB_2161536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15534
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143]. In addition to bands of the predicted molecular mass of 140 kDa, additional PER1 species of 55 and 45 kDa is detected that is predominantly nuclear [PMID: 11726537] .