PES1 Polyclonal antibody

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  WB analysis of COLO 320 using 13553-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13553-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 13553-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:300.

• 

  IHC staining of human gliomas using 13553-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13553-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 13553-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13553-1-AP (PES1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PES1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13553-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PES

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Tested Applications

• Positive WB detected in: COLO 320 cells, MCF-7 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

13553-1-AP targets PES1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4342

  Product name: Recombinant human PES1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 238-588 aa of BC032489

  Sequence: LYQLLNLHYPPKLEGQAQAEAKAGEGTYALDSESCMEKLAALSASLARVVVPATEEEAEVDEFPTDGEMSAQEEDRRKELEAQEKHKKLFEGLKFFLNREVPREALAFIIRSFGGEVSWDKSLCIGATYDVTDSRITHQIVDRPGQQTSVIGRCYVQPQWVFDSVNARLLLPVAEYFSGVQLPPHLSPFVTEKEGDYVPPEKLKLLALQRGEDPGNLNESEEEEEEDDNNEGDGDEEGENEEEEEDAEAGSEKEEEARLAALEEQRMEGKKPRVMAGTLKLEDKQRLAQEEESEAKRLAIMMMKKREKYLYQKIMFGKRRKIREANKLAEKRKAHDEAVRSEKKAKKARPE

• Full Name: pescadillo homolog 1, containing BRCT domain (zebrafish)
• Calculated Molecular Weight: 588 aa, 68 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC032489
• Gene Symbol: PES1
• Gene ID (NCBI): 23481
• RRID: AB_2161967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00541
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Mol Med; Ketogenic diet ameliorates lipid dysregulation in type 2 diabetic mice by downregulating hepatic pescadillo 1.; Authors - Jielin Zhou
human	IHC,WB	Cancer Med; PES1 is a biomarker of head and neck squamous cell carcinoma and is associated with the tumor microenvironment; Authors - Yaodong He
human	WB,IHC,IP	J Exp Clin Cancer Res; SNHG17 promotes colorectal tumorigenesis and metastasis via regulating Trim23-PES1 axis and miR-339-5p-FOSL2-SNHG17 positive feedback loop.; Authors - Zehua Bian
human	WB,IHC	Life Sci; PES1 enhances proliferation and tumorigenesis in hepatocellular carcinoma via the PI3K/AKT pathway.; Authors - Jing Wang ; KD Validated
goat	WB	Int J Mol Sci; Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods; Authors - Zhipeng Sun
rat	IHC	J Inflamm Res; Analysis and Validation of Autophagy-Related Gene Biomarkers and Immune Cell Infiltration Characteristic in Bronchopulmonary Dysplasia by Integrating Bioinformatics and Machine Learning; Authors - Shuzhe Xiao