CoraLite® Plus 488-conjugated PEX14 (human specific) Recombinant antibody
PEX14 (human specific) Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
6K5
Cat no : CL488-80196
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-80196 targets PEX14 (human specific) in FC (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PEX14 (human specific) fusion protein Ag0932 |
Full Name | peroxisomal biogenesis factor 14 |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 57 kDa |
GenBank Accession Number | BC006327 |
Gene Symbol | PEX14 |
Gene ID (NCBI) | 5195 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.
This antibody is specific to human PEX14. It can't detect mouse or rat PEX14.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 PEX14 (human specific) antibody CL488-80196 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |