PEX19 Monoclonal antibody

PEX19 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3H2C2

Cat no : 68555-1-Ig

Synonyms

33 kDa housekeeping protein, D1S2223E, HK33, Peroxin 19, PEX19, PMP1, PMPI, PXF, PXMP1

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Validation Data Gallery

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  WB analysis using 68555-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68555-1-Ig (PEX19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, Pig liver tissue, Rat liver tissue, Mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68555-1-Ig targets PEX19 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PEX19 fusion protein Ag6858
• Full Name: peroxisomal biogenesis factor 19
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC000496
• Gene Symbol: PEX19
• Gene ID (NCBI): 5824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).