PFAS Polyclonal antibody, PBS Only

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• PFAS Polyclonal antibody, PBS Only (24716-1-PBS)

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  WB analysis using 24716-1-AP (same clone as 24716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24716-1-AP (PFAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

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  WB analysis using 24716-1-AP (same clone as 24716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24716-1-AP (PFAS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 24716-1-AP (same clone as 24716-1-PBS)

  WB result of PFAS antibody (24716-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFAS transfected HeLa cells. This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 24716-1-AP (same clone as 24716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24716-1-AP (PFAS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 24716-1-AP (same clone as 24716-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PFAS antibody (24716-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24716-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PFAS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24716-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:6.3.5.3, FGAM synthase, FGAMS, FGAR amidotransferase, FGARAT

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Product Information

24716-1-PBS targets PFAS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20162

  Product name: Recombinant human PFAS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 989-1338 aa of BC167158

  Sequence: MVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPPTFPKASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTSPWLQLSINARNWTLEGSC

• Full Name: phosphoribosylformylglycinamidine synthase
• Calculated Molecular Weight: 1338 aa, 145 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC167158
• Gene Symbol: PFAS
• Gene ID (NCBI): 5198
• RRID: AB_3669446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15067
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.