PFKFB4 Recombinant monoclonal antibody

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  WB analysis using 85444-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85444-3-RR (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85444-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85444-3-RR against Human PFKFB4 were performed. The affinity constant is 0.705 nM.

PFKFB4 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85444-3-RR

Clone No.242962H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242962H8, 6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4

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Tested Applications

• Positive WB detected in: HeLa cells, MOLT-4 cells, PC-3 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85444-3-RR targets PFKFB4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30571

  Product name: Recombinant human PFKFB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 139-232 aa of NM_001317134

  Sequence: ATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVAD

• Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_001317134
• Gene Symbol: PFKFB4
• Gene ID (NCBI): 5210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16877
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.