Tested Applications
| Positive WB detected in | BGC-823 cells, SGC-7901 cells |
| Positive IHC detected in | mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17330-1-AP targets Pepsinogen I in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11309 Product name: Recombinant human PGA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-388 aa of BC029055 Sequence: MKWLLLLGLVALSECIMYKVPLIRKKSLRRTLSERGLLKDFLKKHNLNPARKYFPQWEAPTLVDEQPLENYLDMEYFGTIGIGTPAQDFTVVFDTGSSNLWVPSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGTGSMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQDLFSVYLSADDKSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITMNGETIACAEGCQAIVDTGTSLLTGPTSPIANIQSDIGASENSDGDMVVSCSAISSLPDIVFTINGVQYPVPPSAYILQSEGSCISGFQGMNVPTESGELWILGDVFIRQYFTVFDRANNQVGLAPVA Predict reactive species |
| Full Name | pepsinogen 5, group I (pepsinogen A) |
| Calculated Molecular Weight | 388 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC029055 |
| Gene Symbol | PGA5 |
| Gene ID (NCBI) | 5222 |
| RRID | AB_11042599 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0DJD9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pepsinogen I belongs to the peptidase A1 family. It shows particularly broad specificity and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The gene encodes a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. This antibody can recognize PGA3(pepsinogen 3), PGA4(pepsinogen 4), PGA5(pepsinogen 5).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Pepsinogen I antibody 17330-1-AP | Download protocol |
| WB protocol for Pepsinogen I antibody 17330-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Pepsinogen I Polyclonal antibody (Cat# 17330-1-AP) has 6 citations published in J Agric Food Chem, J Pathol, Hum Pathol, PLoS One, Biochem Biophys Res Commun, and Dev Dyn. Research fields span gastric physiology and chemosensing, gastric stem cell biology, gastric cancer pathology, gastric preneoplasia, neurobiology (choroid plexus and depressive behaviors), and embryonic stomach development involving Hedgehog and Wnt signaling. Applications cited include IHC, IF, and WB across human, mouse, rat, and pig species.
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Casein Hydrolysate Enhances Upper Gastrointestinal Chemosensing and Gastric Acid Secretion in Pigs | ||
Hum Pathol Clinical Significance of Spasmolytic Polypeptide-expressing Metaplasia and Intestinal Metaplasia in EBV-associated and EBV-negative Gastric Cancer. | ||
PLoS One The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival. | ||
Biochem Biophys Res Commun Changed PGA and POSTN levels in choroid plexus are associated with depressive-like behaviors in mice. | ||
Dev Dyn Glypican-6 and Glypican-4 stimulate embryonic stomach growth by regulating Hedgehog and non-canonical Wnt signaling |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody for Western Blot and Immunohistochemistry on both cell and tissue samples, reporting very good and consistent results even at low concentrations. Overall, the feedback highlights the antibody's reliability and strong performance across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FAISAL (Verified Customer) (05-03-2021) | I found this antibody very good and give very good and consistent result in low concentration.
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