Pepsinogen I Polyclonal antibody

Pepsinogen I Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 17330-1-AP

Synonyms

PGA5, PGI, Pepsinogen-5, Pepsin A-5, Pepsin A

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Validation Data Gallery

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  WB analysis using 17330-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17330-1-AP (Pepsinogen I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 17330-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17330-1-AP (Pepsinogen I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 17330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17330-1-AP (Pepsinogen I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 17330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17330-1-AP (Pepsinogen I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: BGC-823 cells, SGC-7901 cells
• Positive IHC detected in: mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

17330-1-AP targets Pepsinogen I in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Pepsinogen I fusion protein Ag11309
• Full Name: pepsinogen 5, group I (pepsinogen A)
• Calculated Molecular Weight: 388 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC029055
• Gene Symbol: PGA5
• Gene ID (NCBI): 5222
• RRID: AB_11042599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pepsinogen I belongs to the peptidase A1 family. It shows particularly broad specificity and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The gene encodes a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. This antibody can recognize PGA3(pepsinogen 3), PGA4(pepsinogen 4), PGA5(pepsinogen 5).

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; Changed PGA and POSTN levels in choroid plexus are associated with depressive-like behaviors in mice.; Authors - Jie Liu
rat	IF	PLoS One; The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival.; Authors - Pål Vange
human	IHC	Hum Pathol; Clinical Significance of Spasmolytic Polypeptide-expressing Metaplasia and Intestinal Metaplasia in EBV-associated and EBV-negative Gastric Cancer.; Authors - Yu Zhang
mouse	IHC	Dev Dyn; Glypican-6 and Glypican-4 stimulate embryonic stomach growth by regulating Hedgehog and non-canonical Wnt signaling; Authors - Wen Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

FAISAL (Verified Customer) (05-03-2021)

I found this antibody very good and give very good and consistent result in low concentration.

• Applications: Western Blot, Immunohistochemistry
• Cell Tissue Type: cell and tissue