PGCP Polyclonal antibody

PGCP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16601-1-AP

Synonyms

Plasma glutamate carboxypeptidase, Lysosomal dipeptidase, LCH1, EC:3.4.17.-, CPQ

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Validation Data Gallery

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  WB analysis of L02 using 16601-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16601-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16601-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 16601-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16601-1-AP

  IP result of anti-PGCP (IP:16601-1-AP, 4ug; Detection:16601-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IF Staining of L02 using 16601-1-AP

  Immunofluorescent analysis of L02 cells using 16601-1-AP (PGCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells, human placenta tissue, human plasma tissue, mouse kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

16601-1-AP targets PGCP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGCP fusion protein Ag9770
• Full Name: plasma glutamate carboxypeptidase
• Calculated Molecular Weight: 472 aa, 52 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC020689
• Gene Symbol: PGCP
• Gene ID (NCBI): 10404
• RRID: AB_2160929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGCP(plasma glutamate carboxypeptidase) is also named as LCH1,CPQ and belongs to the peptidase M28 family. It may play an important role in the hydrolysis of circulating peptides. It catalyzes more efficiently the hydrolysis of dipeptides with unsubstituted terminals into amino acids. It is a proteinase that acts on the unsubstituted N- and C-termini of dipeptides. It has been suggested that this PGCP is involved in the release of thyroxine(PMID:20951214). Human PGCP is a metallopeptidase with five potential glycosylation sites, whose activity depends on dimerizationand it can be detected a band of 65-72kd in FRTL-5 cells which is pro-PGCP(PMID:22127294). It can be N-glycosylated(PMID:10206990). It has the pro-form of 60-65 kDa and mature form of 55 kDa(PMID:20951214).

Publications

Species	Application	Title
human,rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li