PGM1 Monoclonal antibody

PGM1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2G5A8

Cat no : 66105-1-Ig

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1



Tested Applications

Positive WB detected inHepg2 cells, HEK-293 cells
Positive IHC detected inhuman colon tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66105-1-Ig is PGM1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PGM1 fusion protein Ag7294
Full Name phosphoglucomutase 1
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC001756
Gene symbol PGM1
Gene ID (NCBI) 5236
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PGM1 antibody 66105-1-IgDownload protocol
IHC protocol for PGM1 antibody 66105-1-IgDownload protocol
IF protocol for PGM1 antibody 66105-1-IgDownload protocol
Standard Protocols
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