PGM1 Monoclonal antibody

PGM1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G5A8

Cat no : 66105-1-Ig

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1

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Validation Data Gallery

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  WB analysis using 66105-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66105-1-Ig (PGM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 66105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 66105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100.

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  IHC staining of human liver cancer using 66105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100.

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  IF Staining of HepG2 using 66105-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PGM1 antibody (66105-1-Ig, Clone: 2G5A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-231 cells, HepG2 cells, pig liver tissue, rabbit liver tissue, rat liver tissue, mouse liver tissue, pig heart tissue, rabbit heart tissue
• Positive IHC detected in: human colon tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Product Information

66105-1-Ig targets PGM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PGM1 fusion protein Ag7294
• Full Name: phosphoglucomutase 1
• Calculated molecular weight: 61 kDa
• Observed molecular weight: 61 kDa
• GenBank accession number: BC001756
• Gene symbol: PGM1
• Gene ID (NCBI): 5236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.