Tested Applications
| Positive WB detected in | HEK-293 cells, fetal human brain tissue, Jurkat cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11022-1-AP targets PGM2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1488 Product name: Recombinant human PGM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 264-612 aa of BC010087 Sequence: QSAFKAFDLVPPEAVPEQKDPDPEFPTVKYPNPEEGKGVLTLSFALADKTKARIVLANDPDADRLAVAEKQDSGEWRVFSGNELGALLGWWLFTSWKEKNQDRSALKDTYMLSSTVSSKILRAIALKEGFHFEETLTGFKWMGNRAKQLIDQGKTVLFAFEEAIGYMCCPFVLDKDGVSAAVISAELASFLATKNLSLSQQLKAIYVEYGYHITKASYFICHDQETIKKLFENLRNYDGKNNYPKACGKFEISAIRDLTTGYDDSQPDKKAVLPTSKSSQMITFTFANGGVATMRTSGTEPKIKYYAELCAPPGNSDPEQLKKELNELVSAIEEHFFQPQKYNLQPKAD Predict reactive species |
| Full Name | phosphoglucomutase 2 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC010087 |
| Gene Symbol | PGM2 |
| Gene ID (NCBI) | 55276 |
| RRID | AB_2161422 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96G03 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PGM2 antibody 11022-1-AP | Download protocol |
| IP protocol for PGM2 antibody 11022-1-AP | Download protocol |
| WB protocol for PGM2 antibody 11022-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PGM2 Polyclonal antibody (Cat# 11022-1-AP) has 2 citations. They appear in Nature Metabolism (2023) and Heliyon (2024). The research fields covered include ribose salvage and glycolysis under nutrient-limited conditions, as well as nuclear translocation of PGM2 in DNA damage response and anti-tumor immunity. Both studies used the antibody for Western blot, with the latter also employing immunofluorescence, in human samples.
| Species | Application | Title |
|---|---|---|
Nat Metab Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions
| ||
Heliyon Nuclear translocation of nucleotide enzyme Phosphoglucomutase 2 governs DNA damage response and anti-tumor immunity |
Reviews
What customers say
The single review for this product reports a 5-star rating from a Western Blot application on ovarian cancer tissue. The reviewer used a 1:1000 dilution and noted that the antibody worked well with no non-specific bands observed. Overall, the feedback is positive, highlighting clean specificity and reliable performance in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Patricia (Verified Customer) (02-25-2020) | Works well. No non-specific bands.
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